Cellular Effects of Heavy Metals by Gaspar Banfalvi

Author:Gaspar Banfalvi
Language: eng
Format: epub
Publisher: Springer Netherlands, Dordrecht

The chromatin changes seen in murine preB cells also correspond to those seen in CHO cells after Cd treatment. Most typical changes of cadmium treatment are the severe disruptions of the nuclear membrane observed from the mid S phase (Fig. 6.6, elutriation fractions 6–8) till the end of S phase, in G2 and M phases (Fig. 6.6, fractions 10–12, late S). These disruptions did not allow to distinguish among the final stages of chromatin condensation, resulting in nuclei with big holes inside them and sticky imperfectly condensed chromosomes (Fig. 6.6, fraction 12, panel D)

Fig. 6.6Cadmium induced chromatin changes in mid S phase (Elutriation Fraction 6–8) and at the end of S phase nuclei of murine preB cells (Elutriation Fraction 10–12). Cells were treated and chromatin structures were isolated as described in the Methods. Fraction 6–8: large-sized perinuclear granules and holes in the middle of the nucleus (a–d). Elutriation fraction 10: disrupted nuclear membrane with large holes (a–d). Elutriation fraction 12: linear arrangement of condensing chromosomes (a–c), sticky precondensed chromosomes. (d) Bars 5 μm each. (With permission of Banfalvi et al. 2007)

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